Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells.

ABSTRACT: Organoids derived from human pluripotent stem cells are interesting models to study mechanisms of morphogenesis and promising platforms for disease modeling and drug screening. However, they mostly remain incomplete as they lack stroma, tissue resident immune cells and in particular vasculature, which create important niches during development and disease. We propose, that the directed incorporation of mesodermal progenitor cells (MPCs) into organoids will overcome the aforementioned limitations. In order to demonstrate the feasibility of the method, we generated complex human tumor as well as neural organoids. We show that the formed blood vessels display a hierarchic organization and mural cells are assembled into the vessel wall. Moreover, we demonstrate a typical blood vessel ultrastru

SUBMITTER: Worsdorfer P 

PROVIDER: S-EPMC6821804 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature