Details of the cooperative binding of piperlongumine with rat serum albumin obtained by spectroscopic and computational analyses.

ABSTRACT: Piperlongumine (PPL) has presented a variety of important pharmacological activities. In recent pharmacokinetics studies in rats, this molecule reached 76.39% of bioavailability. Although PPL is present in the bloodstream, no information is found on the interaction between PPL and rat serum albumin (RSA), the most abundant protein with the function of transporting endo/exogenous molecules. In this sense, the present study elucidated the mechanism of interaction between PPL and RSA, using in conjunction spectroscopic and computational techniques. This paper shows the importance of applying inner filter correction over the entire fluorescence spectrum prior to any conclusion regarding changes in the polarity of the fluorophore microenvironment, also demonstrates the convergence of the result

SUBMITTER: Povinelli APR 

PROVIDER: S-EPMC6821919 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature