IMP-68, a Novel IMP-Type Metallo-β-Lactamase in Imipenem-Susceptible Klebsiella pneumoniae.

ABSTRACT: We recently detected a novel variant of an IMP-type metallo-β-lactamase gene (bla_IMP-68) from meropenem-resistant but imipenem-susceptible Klebsiella pneumoniae TA6363 isolated in Tokyo, Japan. bla_IMP-68 encodes a Ser262Gly point mutant of IMP-11, and transformation experiments showed that bla_IMP-68 increased the MIC of carbapenems in recipient strains, whereas the MIC of imipenem was not greatly increased relative to that of other carbapenems, including meropenem. Kinetics experiments showed that IMP-68 imipenem-hydrolyzing activity was lower than that for other carbapenems, suggesting that the antimicrobial susceptibility profile of TA6363 originated from IMP-68 substrate specificity. Whole-genome sequencing showed that bla<

SUBMITTER: Kubota H 

PROVIDER: S-EPMC6821933 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature