Targeted High Resolution LC/MS3 Adductomics Method for the Characterization of Endogenous DNA Damage.

ABSTRACT: DNA can be damaged through covalent modifications of the nucleobases by endogenous processes. These modifications, commonly referred to as DNA adducts, can persist and may lead to mutations, and ultimately to the initiation of cancer. A screening methodology for the majority of known endogenous DNA adducts would be a powerful tool for investigating the etiology of cancer and for the identification of individuals at high-risk to the detrimental effects of DNA damage. This idea led to the development of a DNA adductomic approach using high resolution data-dependent scanning, an extensive MS2 fragmentation inclusion list of known endogenous adducts, and neutral loss MS3 triggering to profile all DNA modifications. In this method, the detection of endogenous DNA adducts is performed by observa

SUBMITTER: Carra A 

PROVIDER: S-EPMC6822301 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature