Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France.

ABSTRACT:

Background

We investigate here the presence of multidrug-resistant bacteria isolated from stool samples of yellow-legged gulls and chickens (n = 136) in urban parks and beaches of Marseille, France. Bacterial isolation was performed on selective media, including MacConkey agar with ceftriaxone and LBJMR medium. Antibiotic resistance genes, including extended-spectrum β-lactamases (ESBL) (i.e. bla_CTX-M, bla_TEM and bla_SHV), carbapenemases (bla_KPC, bla_VIM, bla_NDM, bla_OXA-23, bla_OXA-24, bla_OXA-48 and bla_OXA-58) and colistin resistance genes (mcr-1 to mcr-5) were screened by real-time PCR and standard PCR and sequenced when found.

Results

Of the 136 stools samples collect

SUBMITTER: Ngaiganam EP 

PROVIDER: S-EPMC6822345 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature