Microevolution within ST11 group Clostridioides difficile isolates through mobile genetic elements based on complete genome sequencing.

ABSTRACT:

Background

Clade 5 Clostridioides difficile diverges significantly from the other clades and is therefore, attracting increasing attention due its great heterogeneity. In this study, we used third-generation sequencing techniques to sequence the complete whole genomes of three ST11 C. difficile isolates, RT078 and another two new ribotypes (RTs), obtained from three independent hospitalized elderly patients undergoing antibiotics treatment. Mobile genetic elements (MGEs), antibiotic-resistance, drug resistance genes, and virulent-related genes were analyzed and compared within these three isolates.

Results

Isolates 10,010 and 12,038 carried a distinct deletion in tcdA compared with isolate 21,062. Furthermore, all three isolates had identical deletions and point-mutations in

SUBMITTER: Wu Y 

PROVIDER: S-EPMC6822371 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature