Cooperative evolution of two different TEs results in lineage-specific novel transcripts in the BLOC1S2 gene.

ABSTRACT:

Background

The BLOC1S2 gene encodes the multifunctional protein BLOS2, a shared subunit of two lysosomal trafficking complexes: i) biogenesis of lysosome-related organelles complex-1 and i) BLOC-1-related complex. In our previous study, we identified an intriguing unreported transcript of the BLOC1S2 gene that has a novel exon derived from two transposable elements (TEs), MIR and AluSp. To investigate the evolutionary footprint and molecular mechanism of action of this transcript, we performed PCR and RT-PCR experiments and sequencing analyses using genomic DNA and RNA samples from humans and various non-human primates.

Results

The results showed that the MIR element had integrated into the genome of our common ancestor, specifically in the BLOC1S2 gene region, before the ra

SUBMITTER: Cho HM 

PROVIDER: S-EPMC6822395 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature