Complete nontuberculous mycobacteria whole genomes using an optimized DNA extraction protocol for long-read sequencing.

ABSTRACT:

Background

Nontuberculous mycobacteria (NTM) are a major cause of pulmonary and systemic disease in at-risk populations. Gaps in knowledge about transmission patterns, evolution, and pathogenicity during infection have prompted a recent surge in genomic NTM research. Increased availability and affordability of whole genome sequencing (WGS) techniques provide new opportunities to sequence and construct complete bacterial genomes faster and at a lower cost. However, extracting large quantities of pure genomic DNA is particularly challenging with NTM due to its slow growth and recalcitrant cell wall. Here we report a DNA extraction protocol that is optimized for long-read WGS of NTM, yielding large quantities of highly pure DNA with no additional clean-up steps.

Results

Our DNA

SUBMITTER: Bouso JM 

PROVIDER: S-EPMC6822416 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature