CD147 as a key mediator of the spleen inflammatory response in mice after focal cerebral ischemia.

ABSTRACT: BACKGROUND:The splenic inflammatory response after cerebral ischemia has been implicated in secondary brain injury. We have recently reported that CD147 plays an important role in driving brain inflammation after ischemic stroke. In this study, we hypothesized that CD147 may play a role in the splenic inflammatory response after cerebral ischemia. METHODS:Transient (60 min) middle cerebral artery occlusion was induced in wild-type mice treated with an anti-CD147 antibody (αCD147) 1 h before ischemia onset. The splenic inflammatory response was evaluated at 4 and 24 h, representing the peak and early stage of splenic inflammatory activation in this model. Changes in mRNA and protein expression of CD147 and inflammatory markers were measured using RT-qPCR and western blot, respectively. Immu

SUBMITTER: Jin R 

PROVIDER: S-EPMC6822438 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature