Isolation and molecular characterization of dengue virus clinical isolates from pediatric patients in New Delhi.

ABSTRACT:

Objective

To characterize the in vitro replication fitness, viral diversity, and phylogeny of dengue viruses (DENV) isolated from Indian patients.

Methods

DENV was isolated from whole blood collected from patients by passaging in cell culture. Passage 3 viruses were used for growth kinetics in C6/36 mosquito cells. Parallel efforts also focused on the isolation of DENV RNA from plasma samples of the same patients, which were processed for next-generation sequencing.

Results

It was possible to isolate 64 clinical isolates of DENV, mostly DENV-2. Twenty-five of these were further used for growth curve analysis in vitro, which showed a wide range of replication kinetics. The highest viral titers were associated with isolates from patients with dengue with warning signs a

SUBMITTER: Kar M 

PROVIDER: S-EPMC6823047 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature