Effect of the vaginal microbiome on the pregnancy rate in women receiving assisted reproductive treatment.

ABSTRACT:

Purpose

To investigate if the vaginal microbiome influences the IVF outcome.

Methods

Thirty-one patients undergoing assisted reproductive treatment (ART) with own or donated gametes and with cryotransfer of a single euploid blastocyst were recruited for this cohort study. Two vaginal samples were taken during the embryo transfer procedure, just before transferring the embryo. The V3 V4 region of 16S rRNA was used to analyze the vaginal microbiome, and the bioinformatic analysis was performed using QIIME2, Bioconductor Phyloseq, and MicrobiomeAnalyst packages. Alpha diversity was compared between groups according to the result of the pregnancy test.

Results

Fourteen (45.2%) patients did not and seventeen (54.8 %) did achieve pregnancy under ART. A greater index of alph

SUBMITTER: Bernabeu A 

PROVIDER: S-EPMC6823330 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature