Removal of DNA-fragmented spermatozoa using flow cytometry and sorting does not improve the outcome of intracytoplasmic sperm injection.

ABSTRACT:

Purpose

The DNA fragmentation in sperm is associated with reduced outcome in assisted reproduction. Using YoPro1 as the staining dye and flow cytometry and sorting (FACS), the number of spermatozoa with DNA fragmentation can be lowered to 5%. Can the cumulative outcome of ICSI be improved using FACS?

Methods

A prospective, randomized, double-blind clinical trial was conducted in 104 infertile couples with male infertility based on abnormal conventional semen analysis results. Cumulative ongoing pregnancy rate was the primary outcome parameter. In 52 cases, semen was processed for ICSI using swim-up. In another 52 cases, spermatozoa with fragmented DNA were removed with FACS.

Results

The cumulative pregnancy rate at 12 weeks of gestation (51.9% versus 46.2%) and live b

SUBMITTER: De Geyter C 

PROVIDER: S-EPMC6823333 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature