Gene expression analysis of ovine prepubertal testicular tissue vitrified with a novel cryodevice (E.Vit).

ABSTRACT:

Purpose

Testicular tissue cryopreservation prior to gonadotoxic therapies is a method to preserve fertility in children. However, the technique still requires development, especially when the tissue is immature and rather susceptible to stress derived from in vitro manipulation. This study aimed to investigate the effects of vitrification with a new cryodevice (E.Vit) on cell membrane integrity and gene expression of prepubertal testicular tissue in the ovine model.

Methods

Pieces of immature testicular tissue (1 mm³) were inserted into "E.Vit" devices and vitrified with a two-step protocol. After warming, tissues were cultured in vitro and cell membrane integrity was assessed after 0, 2, and 24 h by trypan blue exclusion test. Controls consisted of non-vitrified

SUBMITTER: Bebbere D 

PROVIDER: S-EPMC6823412 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature