Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells.

ABSTRACT: The gingival epithelium is a physical and immunological barrier to the microbiota of the oral cavity, which interact through soluble mediators with the immune cells that patrol the tissue at the gingival epithelium. We sought to develop a three-dimensional gingivae-biofilm interface model using a commercially available gingival epithelium to study the tissue inflammatory response to oral biofilms associated with "health", "gingivitis" and "periodontitis". These biofilms were developed by sequential addition of microorganisms to mimic the formation of supra- and sub-gingival plaque in vivo. Secondly, to mimic the interactions between gingival epithelium and immune cells in vivo, we integrated peripheral blood mononuclear cells and CD14⁺ monocytes into our three-dimensional model

SUBMITTER: Brown JL 

PROVIDER: S-EPMC6823452 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature