Paper Stacks for Uniform Rehydration of Dried Reagents in Paper Microfluidic Devices.

ABSTRACT: Spatially uniform reconstitution of dried reagents is critical to the function of paper microfluidic devices. Advancing fluid fronts in paper microfluidic devices drive (convect) and concentrate rehydrated reagents to the edges, causing steep chemical gradients and imperfect mixing. This largely unsolved problem in paper microfluidics is exacerbated by increasing device dimensions. In this article, we demonstrate that mixing of dried reagents with a rehydrating fluid in paper microfluidics may be significantly enhanced by stacking paper layers having different wicking rates. Compared to single-layer paper membranes, stacking reduced the "non-reactive area", i.e. area in which the reconstituted reagents did not interact with the rehydrating fluid, by as much as 97% in large (8 cm × 2 cm) pa

SUBMITTER: Das D 

PROVIDER: S-EPMC6823543 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature