PLIN2 Functions As a Novel Link Between Progesterone Signaling and Metabolism in Uterine Leiomyoma Cells.

ABSTRACT: CONTEXT:Uterine leiomyoma (fibroids) are the most common tumors in women. Recently, perilipin-2 (PLIN2) was identified as a critical target gene of the progesterone receptor; however, its function in the pathogenesis of fibroids is unknown. OBJECTIVE:To determine the function of PLIN2 in leiomyoma cells. DESIGN:Tissue and primary cells from leiomyoma and myometrium were analyzed. PLIN2 function in leiomyoma was assessed using small interfering RNA. RNA-sequencing was performed to identify genome-wide effects of PLIN2 depletion. Metabolic activity was measured using the Seahorse XF96 analyzer. Real-time quantitative PCR and immunoblotting were also performed. SETTING:Laboratory. PATIENTS OR OTHER PARTICIPANTS:Forty-one premenopausal women undergoing surgery for fibroids. MAIN OUTCOME MEASUR

SUBMITTER: Okeigwe I 

PROVIDER: S-EPMC6823729 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature