Electrochemical Studies of Cation Condensation-Induced Collapse of Surface-Bound DNA.

ABSTRACT: In this paper, we demonstrate the ability to control and electrochemically monitor nucleic acid conformation by inducing collapse of short, surface-bound nucleotides (7-28 nucleotides). More specifically, we monitored changes in a 5'-electrode-bound DNA structure via changes in the faradaic current related to the reduction/oxidation of a 3'-terminal-appended redox molecule. Reversible DNA collapse was induced by cation condensation achieved by either reducing the dielectric permittivity of the interrogation solution or by the addition of multivalent cations such as the polyamine spermidine (3⁺). Additionally, we find that while the change in electrochemical signal associated with surface bound DNA collapse is dependent on nucleic acid length and surface packing density, the solu

SUBMITTER: Sykes KS 

PROVIDER: S-EPMC6823840 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature