Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples.

ABSTRACT: Protein N termini unambiguously identify truncated, alternatively translated or modified proteoforms with distinct functions and reveal perturbations in disease. Selective enrichment of N-terminal peptides is necessary to achieve proteome-wide coverage for unbiased identification of site-specific regulatory proteolytic processing and protease substrates. However, many proteolytic processes are strictly confined in time and space and therefore can only be analyzed in minute samples that provide insufficient starting material for current enrichment protocols. Here we present High-efficiency Undecanal-based N Termini EnRichment (HUNTER), a robust, sensitive and scalable method for the analysis of previously inaccessible microscale samples. HUNTER achieved identification of >1000 N termini fro

SUBMITTER: Weng SSH 

PROVIDER: S-EPMC6823850 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature