Hi-C guided assemblies reveal conserved regulatory topologies on X and autosomes despite extensive genome shuffling.

ABSTRACT: Genome rearrangements that occur during evolution impose major challenges on regulatory mechanisms that rely on three-dimensional genome architecture. Here, we developed a scaffolding algorithm and generated chromosome-length assemblies from Hi-C data for studying genome topology in three distantly related Drosophila species. We observe extensive genome shuffling between these species with one synteny breakpoint after approximately every six genes. A/B compartments, a set of large gene-dense topologically associating domains (TADs), and spatial contacts between high-affinity sites (HAS) located on the X chromosome are maintained over 40 million years, indicating architectural conservation at various hierarchies. Evolutionary conserved genes cluster in the vicinity of HAS, while HAS

SUBMITTER: Renschler G 

PROVIDER: S-EPMC6824461 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature