Lipid species affect morphology of endoplasmic reticulum: a sea urchin oocyte model of reversible manipulation.

ABSTRACT: The ER is a large multifunctional organelle of eukaryotic cells. Malfunction of the ER in various disease states, such as atherosclerosis, diabetes, cancer, Alzheimer's and Parkinson's and amyotrophic lateral sclerosis, often correlates with alterations in its morphology. The ER exhibits regionally variable membrane morphology that includes, at the extremes, large relatively flat surfaces and interconnected tubular structures highly curved in cross-section. ER morphology is controlled by shaping proteins that associate with membrane lipids. To investigate the role of these lipids, we developed a sea urchin oocyte model, a relatively quiescent cell in which the ER consists mostly of tubules. We altered levels of endogenous diacylglycerol (DAG), phosphatidylethanolamine (PtdEth), and phospha

SUBMITTER: Ulloa G 

PROVIDER: S-EPMC6824487 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature