A new method for measuring cholesterol efflux capacity uses stable isotope-labeled, not radioactive-labeled, cholesterol.

ABSTRACT: The incidence of cardiovascular events correlates inversely with cholesterol efflux capacity (CEC) more than with HDL-cholesterol level. The measurement of CEC is used to qualify cardiovascular disease risk and is conventionally performed with radioisotope (RI)-labeled cholesterol. Here, we established a CEC measurement technique using stable isotope-labeled cholesterol as an alternative, and we compared the new method with RI and fluorescence (boron dipyrromethene difluoride-cholesterol) methods in cells and in patient serum. We incubated J774 cells labeled with [d ₇]cholesterol ([d ₇]C) with patient serum depleted of apoB, and [d ₇]C extracted from the culture medium was quantified by liquid chromatography/quadrupole time-of-flight ma

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC6824490 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature