The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue.

ABSTRACT: Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).

SUBMITTER: Voigt FF 

PROVIDER: S-EPMC6824906 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature