Mechanisms of vascular permeability and remodeling associated with hemarthrosis in factor VIII-deficient mice.

ABSTRACT:

Background

Vascular remodeling associated with hemophilic arthropathy (HA) may contribute to bleed propagation, but the mechanisms remain poorly understood.

Objectives

To explore molecular mechanisms of HA and the effects of hemostasis correction on synovial vascular remodeling after joint injury in hypocoagulable mice.

Methods

Factor VIII (FVIII)-deficient mice +/- FVIII treatment and hypocoagulable wild-type mice (^Hypo BALB/c) were subjected to subpatellar puncture. ^Hypo BALB/c mice were treated with warfarin and anti-FVIII before injury, after which warfarin was continued for 2 weeks or reversed +/- continuous anti-FVIII until harvest. Synovial vascularity was analyzed at baseline and 2 to 4 weeks post injury by histology, musculoskeletal ultr

SUBMITTER: Cooke EJ 

PROVIDER: S-EPMC6824926 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature