2018 YPIC Challenge: A Case Study in Characterizing an Unknown Protein Sample.

ABSTRACT: For the 2018 YPIC Challenge, contestants were invited to try to decipher two unknown English questions encoded by a synthetic protein expressed in Escherichia coli. In addition to deciphering the sentence, contestants were asked to determine the three-dimensional structure and detect any post-translation modifications left by the host organism. We present our experimental and computational strategy to characterize this sample by identifying the unknown protein sequence and detecting the presence of post-translational modifications. The sample was acquired with dynamic exclusion disabled to increase the signal-to-noise ratio of the measured molecules, after which spectral clustering was used to generate high-quality consensus spectra. De novo spectrum identification was used to deter

SUBMITTER: Pino L 

PROVIDER: S-EPMC6824964 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature