Epitope peptides of Helicobacter pylori CagA antibodies from sera by whole-peptide mapping.

ABSTRACT:

Background

Helicobacter pylori CagA has been found to be immuno-dominant protein and utilized for the diagnosis of the infection with cagA-positive strains. It is important to characterize the peptide epitopes capable of detecting serum anti-CagA antibodies to understand CagA immunogenicity.

Methods

Sera from 171 Japanese patients were subjected for the epitope mapping study. Eighty seven peptides were designed from the CagA consensus sequence and were used for ELISA protocol to test the serum samples. The reacting anti-CagA IgG amounts to specific peptides were measured and compared.

Results

The study revealed a strong reactivity of two peptides (c7-NNTEPIYAQVNKKKAGQAT and c8-AGQATSPEEPIYAQVAKKV) in H. pylori-infected group. Interestingly, these two peptides containe

SUBMITTER: Ansari S 

PROVIDER: S-EPMC6824978 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature