Serum-deprived differentiated neuroblastoma F-11 cells express functional dorsal root ganglion neuron properties.

ABSTRACT: The isolation and culture of dorsal root ganglion (DRG) neurons cause adaptive changes in the expression and regulation of ion channels, with consequences on neuronal excitability. Considering that not all neurons survive the isolation and that DRG neurons are heterogeneous, it is difficult to find the cellular subtype of interest. For this reason, researchers opt for DRG-derived immortal cell lines to investigate endogenous properties. The F-11 cell line is a hybridoma of embryonic rat DRG neurons fused with the mouse neuroblastoma line N18TG2. In the proliferative condition, F-11 cells do not display a gene expression profile correspondent with specific subclasses of sensory neurons, but the most significant differences when compared with DRGs are the reduction of voltage-gated sodium, p

SUBMITTER: Pastori V 

PROVIDER: S-EPMC6825413 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature