Analysis of somatic mutations identifies signs of selection during in vitro aging of primary dermal fibroblasts.

ABSTRACT: Somatic mutations are critical for cancer development and may play a role in age-related functional decline. Here, we used deep sequencing to analyze the prevalence of somatic mutations during in vitro cell aging. Primary dermal fibroblasts from healthy subjects of young and advanced age, from Hutchinson-Gilford progeria syndrome and from xeroderma pigmentosum complementation groups A and C, were first restricted in number and then expanded in vitro. DNA was obtained from cells pre- and post-expansion and sequenced at high depth (1656× mean coverage), over a cumulative 290 kb target region, including the exons of 44 aging-related genes. Allele frequencies of 58 somatic mutations differed between the pre- and post-cell culture expansion passages. Mathematical modeling revealed that the freq

SUBMITTER: Narisu N 

PROVIDER: S-EPMC6826141 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature