Nucleotide variations of TP53 exon 4 found in intracranial meningioma and in silico prediction of their significance.

ABSTRACT: The aim of the present study was to identify TP53 exon 4 mutations in patients with meningioma and to investigate their potential association with specific tumor pathology. Nucleotide alterations were investigated in 48 meningiomas via the direct sequencing of TP53 exon 4 in patient tumor and blood samples using the DNA Sanger method with the BigDyeTerminator v3.1 Cycle Sequencing kit and Applied Biosystems 3730XL apparatus. The results revealed that TP53 exon 4 was frequently altered in meningioma, occurring in 60.4% of the patients investigated. A total of 18 different alterations were detected in the meningioma samples assessed in the current study. The majority of these appeared more than once and some were repeatedly identified in several patients. Changes at codo

SUBMITTER: Bukovac A 

PROVIDER: S-EPMC6826266 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature