Transcriptome Profiling Reveals Matrisome Alteration as a Key Feature of Ovarian Cancer Progression.

ABSTRACT:

Background

Ovarian cancer is the most lethal gynecologic malignancy. There is a lack of comprehensive investigation of disease initiation and progression, including gene expression changes during early metastatic colonization.

Methods

RNA-sequencing (RNA-seq) was done with matched primary tumors and fallopian tubes (n = 8 pairs) as well as matched metastatic and primary tumors (n = 11 pairs) from ovarian cancer patients. Since these are end point analyses, it was combined with RNA-seq using high-grade serous ovarian cancer cells seeded on an organotypic three-dimensional (3D) culture model of the omentum, mimicking early metastasis. This comprehensive approach revealed key changes in gene expression occurring in ovarian cancer initiation and metastasis, including early metas

SUBMITTER: Mitra S 

PROVIDER: S-EPMC6826756 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature