^99mTc-A1 as a Novel Imaging Agent Targeting Mesothelin-Expressing Pancreatic Ductal Adenocarcinoma.

ABSTRACT: Mesothelin is a membrane-associated protein overexpressed in pancreatic ductal adenocarcinoma (PDAC). Some mesothelin-targeted therapies are in clinical development but the identification of patients eligible for such therapies is still challenging. The objective of this study was to perform the imaging of mesothelin in mice models of PDAC with a technetium-labeled anti-mesothelin single-domain antibody (^99mTc-A1).

Methods

The Cancer Genomic Atlas (TCGA) database was used to determine the prognostic role of mesothelin in PDAC. ^99mTc-A1 was evaluated both in vitro in PDAC cells (SW1990 and AsPC-1) and in vivo in an experimental model of mesothelin-expressing PDAC (AsPC-1) in mice.

Results

TCGA analysis showed that PDAC patients with high mesothelin expres

SUBMITTER: Montemagno C 

PROVIDER: S-EPMC6827014 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature