PCu_AC domains from methane-oxidizing bacteria use a histidine brace to bind copper.

ABSTRACT: Copper is critically important for methanotrophic bacteria because their primary metabolic enzyme, particulate methane monooxygenase (pMMO), is copper-dependent. In addition to pMMO, many other copper proteins are encoded in the genomes of methanotrophs, including proteins that contain periplasmic copper-Achaperone (PCu_AC) domains. Using bioinformatics analyses, we identified three distinct classes of PCu_AC domain-containing proteins in methanotrophs, termed PmoF1, PmoF2, and PmoF3. PCu_AC domains from other types of bacteria bind a single Cu(I) ion via an HX_nMX_21/22HXM motif, which is also present in PmoF3, but PmoF1 and PmoF2 lack this motif entirely. Instead, the PCu_AC domai

SUBMITTER: Fisher OS 

PROVIDER: S-EPMC6827282 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature