Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments.

ABSTRACT: A third of the genes in prokaryotic and eukaryotic genomes encode membrane proteins that are either essential for signal transduction and solute transport or function as scaffold structures. Unlike many of their soluble counterparts, the overall structural and functional organization of membrane proteins is sparingly understood. Recent advances in X-ray crystallography, cryo-EM, and nuclear magnetic resonance (NMR) are closing this gap by enabling an in-depth view of these ever-elusive proteins at atomic resolution. Despite substantial technological advancements, however, the overall proportion of membrane protein entries in the Protein Data Bank (PDB) remains <4%. This paucity is mainly attributed to difficulties associated with their expression and purification, propensity to form large

SUBMITTER: Puthenveetil R 

PROVIDER: S-EPMC6827292 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature