Molecular identification of HSPA8 as an accessory protein of a hyperpolarization-activated chloride channel from rat pulmonary vein cardiomyocytes.

ABSTRACT: Pulmonary veins (PVs) are the major origin of atrial fibrillation. Recently, we recorded hyperpolarization-activated Cl^- current (I _{Cl, h}) in rat PV cardiomyocytes. Unlike the well-known chloride channel protein 2 (CLCN2) current, the activation curve of I _{Cl, h} was hyperpolarized as the Cl^- ion concentration ([Cl^-] _i ) increased. This current could account for spontaneous activity in PV cardiomyocytes linked to atrial fibrillation. In this study, we aimed to identify the channel underlying I _{Cl, h} Using RT-PCR amplification specific for Clcn2 or its homologs, a chloride channel was cloned from rat PV and detected in rat PV cardiomyocytes using immunocytochemistry. The gene seque

SUBMITTER: Okamoto Y 

PROVIDER: S-EPMC6827309 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature