Sonic hedgehog enhances calcium oscillations in hippocampal astrocytes.

ABSTRACT: Sonic hedgehog (SHH) is important for organogenesis during development. Recent studies have indicated that SHH is also involved in the proliferation and transformation of astrocytes to the reactive phenotype. However, the mechanisms underlying these are unknown. Involvement of SHH signaling in calcium (Ca) signaling has not been extensively studied. Here, we report that SHH and Smoothened agonist (SAG), an activator of the signaling receptor Smoothened (SMO) in the SHH pathway, activate Ca oscillations in cultured murine hippocampal astrocytes. The response was rapid, on a minute time scale, indicating a noncanonical pathway activity. Pertussis toxin blocked the SAG effect, indicating an involvement of a G_i coupled to SMO. Depletion of extracellular ATP by apyrase, an ATP-degrad

SUBMITTER: Adachi C 

PROVIDER: S-EPMC6827318 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature