Modulating Mistranslation Potential of tRNA^Ser in Saccharomyces cerevisiae.

ABSTRACT: Transfer RNAs (tRNAs) read the genetic code, translating nucleic acid sequence into protein. For tRNA^Ser the anticodon does not specify its aminoacylation. For this reason, mutations in the tRNA^Ser anticodon can result in amino acid substitutions, a process called mistranslation. Previously, we found that tRNA^Ser with a proline anticodon was lethal to cells. However, by incorporating secondary mutations into the tRNA, mistranslation was dampened to a nonlethal level. The goal of this work was to identify second-site substitutions in tRNA^Ser that modulate mistranslation to different levels. Targeted changes to putative identity elements led to total loss of tRNA function or significantly impaired cell growth. However, through genetic selection, we

SUBMITTER: Berg MD 

PROVIDER: S-EPMC6827378 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature