Key Genes And Pathways Controlled By E2F1 In Human Castration-Resistant Prostate Cancer Cells.

ABSTRACT:

Background

Treatment of castration-resistant prostate cancer (CRPC) is an enormous challenge. As E2F transcription factor 1 (E2F1) is an essential factor in CRPC, this study investigated the genes and pathways controlled by E2F1 and their effects on cellular behavior in CRPC.

Methods

In vitro assays were used to evaluate cellular proliferation, apoptosis, and behavior. Cellular expression was quantified by RNA sequencing (RNA-seq). Gene co-expression was assessed using the GeneMANIA database, and correlations were analyzed with the GEPIA server. Altered pathways of differentially expressed genes (DEGs) were revealed by functional annotation. Module analysis was performed using the STRING database and hub genes were filtered with the Cytoscape software. Some DEGs were validat

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC6827506 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature