Molecular characterisation of genital human papillomavirus among women in Southwestern, Nigeria.

ABSTRACT: BACKGROUND:Persistent infections with high-risk genital Human papillomavirus (HPV) especially types 16 and 18, are associated with cervical cancer. However, distribution of HPV types varies greatly across geographical regions and the available vaccines target only few types. This study was designed to determine the HPV types circulating in Southwestern Nigeria, thereby providing necessary information for effective control of the virus. METHODS:Endocervical swab samples were collected from a total of 295 consenting women attending routine cervical cancer screening, STI clinics and community-based outreach programme. Viral DNA was extracted from the samples and the consensus region of the HPV DNA was amplified by PCR using GP-E6/E7 primers. Type-specific nested multiplex PCR and Sanger seque

SUBMITTER: Nejo YT 

PROVIDER: S-EPMC6827905 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature