Development of a new largely scalable in vitro prion propagation method for the production of infectious recombinant prions for high resolution structural studies.

ABSTRACT: The resolution of the three-dimensional structure of infectious prions at the atomic level is pivotal to understand the pathobiology of Transmissible Spongiform Encephalopathies (TSE), but has been long hindered due to certain particularities of these proteinaceous pathogens. Difficulties related to their purification from brain homogenates of disease-affected animals were resolved almost a decade ago by the development of in vitro recombinant prion propagation systems giving rise to highly infectious recombinant prions. However, lack of knowledge about the molecular mechanisms of the misfolding event and the complexity of systems such as the Protein Misfolding Cyclic Amplification (PMCA), have limited generating the large amounts of homogeneous recombinant prion preparations required for

SUBMITTER: Erana H 

PROVIDER: S-EPMC6827918 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature