Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin.

ABSTRACT: Bioluminescence imaging (BLI) is ubiquitous in scientific research for the sensitive tracking of biological processes in small animal models. However, due to the attenuation of visible light by tissue, and the limited set of near-infrared bioluminescent enzymes, BLI is largely restricted to monitoring single processes in vivo. Here we show, that by combining stabilised colour mutants of firefly luciferase (FLuc) with the luciferin (LH₂) analogue infraluciferin (iLH₂), near-infrared dual BLI can be achieved in vivo. The X-ray crystal structure of FLuc with a high-energy intermediate analogue, 5'-O-[N-(dehydroinfraluciferyl)sulfamoyl] adenosine (iDLSA) provides insight into the FLuc-iLH₂ reaction leading to near-infrared light emission. The spectral character

SUBMITTER: Stowe CL 

PROVIDER: S-EPMC6828332 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature