Rewiring of Metabolic Network in Mycobacterium tuberculosis During Adaptation to Different Stresses.

ABSTRACT: Metabolic adaptation of Mycobacterium tuberculosis (M. tuberculosis) to microbicidal intracellular environment of host macrophages is fundamental to its pathogenicity. However, an in-depth understanding of metabolic adjustments through key reaction pathways and networks is limited. To understand how such changes occur, we measured the cellular metabolome of M. tuberculosis subjected to four microbicidal stresses using liquid chromatography-mass spectrometric multiple reactions monitoring (LC-MRM/MS). Overall, 87 metabolites were identified. The metabolites best describing the separation between stresses were identified through multivariate analysis. The coupling of the metabolite measurements with existing genome-scale metabolic model, and using constraint-based simula

SUBMITTER: Rizvi A 

PROVIDER: S-EPMC6828651 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature