Expression, Localization, and Effect of High Salt Intake on Electroneutral Na⁺/HCO₃ ^- Cotransporter NBCn2 in Rat Small Intestine: Implication in Intestinal NaCl Absorption.

ABSTRACT: The electroneutral Na⁺/HCO₃ ^- cotransporter NBCn2 (SLC4A10) of solute carrier family 4 (SLC4) plays important physiological and pathological roles in the body. Our previous study showed that NBCn2 is expressed on the protein level in the small intestine of rat. Here, by reverse-transcription polymerase chain reaction (PCR), we identified a novel full-length NBCn2 variant, i.e., NBCn2-K, from rat small intestine. By pH_i measurement with Xenopus oocytes, the activity of NBCn2-K is not significantly different from NBCn2-G. By western blotting, NBCn2 and the Na⁺/H⁺ exchanger NHE3 (SLC9A3) are predominantly expressed in the jejunum of rat small intestine. By immunofluorescence, NBCn2 and NHE3 are localized at the apical do

SUBMITTER: Wang JL 

PROVIDER: S-EPMC6828735 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature