Accessing Methyl Groups in Proteins via ¹H-detected MAS Solid-state NMR Spectroscopy Employing Random Protonation.

ABSTRACT: We recently introduced RAP (reduced adjoining protonation) labelling as an easy to implement and cost-effective strategy to yield selectively methyl protonated protein samples. We show here that even though the amount of H₂O employed in the bacterial growth medium is rather low, the intensities obtained in MAS solid-state NMR ¹H,¹³C correlation spectra are comparable to spectra obtained for samples in which α-ketoisovalerate was employed as precursor. In addition to correlations for Leu and Val residues, RAP labelled samples yield also resonances for all methyl containing side chains. The labelling scheme has been employed to quantify order parameters, together with the respective asymmetry parameters. We obtain a very good correlation between the order par

SUBMITTER: Asami S 

PROVIDER: S-EPMC6828780 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature