Nanobody-based sandwich reporter system for living cell sensing influenza A virus infection.

ABSTRACT: The influenza epidemic is a huge burden to public health. Current influenza vaccines provide limited protection against new variants due to frequent mutation of the virus. The continual emergence of novel variants necessitates the method rapidly monitoring influenza virus infection in experimental systems. Although several replication-competent reporter viruses carrying fluorescent proteins or small luciferase have been generated in previous studies, visualizing influenza virus infection via such strategy requires reverse genetic modification for each viral strain which is usually time-consuming and inconvenient. Here, we created a novel influenza A nucleoprotein (NP) dependent reporter gene transcription activation module using NP-specific nanobodies. Our results demonstrated the modular

SUBMITTER: Cao J 

PROVIDER: S-EPMC6828950 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature