Recombinant Thrombomodulin Suppresses Histone-Induced Neutrophil Extracellular Trap Formation.

ABSTRACT: Histones, the major protein components of chromatin, are released into the extracellular space during sepsis, trauma, and ischemia-reperfusion injury, and subsequently mediate organ failure. Extracellular histones can promote endothelial damage and platelet aggregation, which can be suppressed by administration of recombinant thrombomodulin (rTM). The present study aimed to clarify whether histones can activate neutrophils to induce NET formation and whether rTM can prevent histone-induced NET formation. NET formation was analyzed in vitro by stimulating human neutrophils with histones in the absence or presence of rTM. NET formation was further analyzed in vivo by intravenous infusion of histones into rats with or without rTM. Histones induced NET release in a dose-dependent

SUBMITTER: Shrestha B 

PROVIDER: S-EPMC6828967 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature