Multiple Changes Underlie Allelic Divergence of CUP2 Between Saccharomyces Species.

ABSTRACT: Under the model of micromutationism, phenotypic divergence between species is caused by accumulation of many small-effect changes. While mapping the causal changes to single nucleotide resolution could be difficult for diverged species, genetic dissection via chimeric constructs allows us to evaluate whether a large-effect gene is composed of many small-effect nucleotide changes. In a previously described non-complementation screen, we found an allele difference of CUP2, a copper-binding transcription factor, underlies divergence in copper resistance between Saccharomyces cerevisiae and S. uvarum Here, we tested whether the allele effect of CUP2 was caused by multiple nucleotide changes. By analyzing chimeric constructs containing four separate regions in the

SUBMITTER: Li XC 

PROVIDER: S-EPMC6829129 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature