A Screen of the Conserved Kinome for Negative Regulators of LIN-12 Negative Regulatory Region ("NRR")-Missense Activity in Caenorhabditis elegans.

ABSTRACT: Genetic analysis of LIN-12/Notch signaling in C . elegans has provided many insights into human biology. Activating missense mutations in the Negative Regulatory Region (NRR) of the ectodomain of LIN-12/Notch were first described in C. elegans, and similar mutations in human Notch were later found to cause T-cell acute lymphoblastic leukemia (T-ALL). The ubiquitin ligase sel-10 /Fbw7 is the prototype of a conserved negative regulator of lin-12 /Notch that was first defined by loss-of-function mutations that enhance lin-12 NRR-missense activity in C. elegans, and then demonstrated to regulate Notch activity in mammalian cells and to be a bona fide tumor suppressor in T-ALL. Here, we report the results of an RNAi sc

SUBMITTER: Deng Y 

PROVIDER: S-EPMC6829150 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature