Fusobacterium nucleatum prevents apoptosis in colorectal cancer cells via the ANO1 pathway.

ABSTRACT:

Objective

: Chemotherapy failure derived from drug resistance is the most important reason causing the recurrence in colorectal cancer patients. Therefore, it is necessary to shed light on the mechanism of chemotherapy resistance in colorectal cancer patients.

Methods

: We looked into the contribution of Fusobacterium nucleatum and ANO1 to chemoresistance in the human colorectal carcinoma cell lines. We silence and overexpress ANO1 in HCT116 and HT29 cells with lentivirus and siRNA knockdown technique in the absence or presence of F. nucleatum, oxaliplatin or 5-fluorouracil (5-FU). ANO1, p-pg, cleaved PARP, cleaved caspase-3, and EGFR expression was measured by Western blot. Cell apoptosis was measured by flow cytometry.

Results

: We found that

SUBMITTER: Lu P 

PROVIDER: S-EPMC6829176 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature