Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae.

ABSTRACT: Conjugation is a bacterial mechanism for DNA transfer from a donor cell to a wide range of recipients, including both prokaryotic and eukaryotic cells. In contrast to conventional DNA delivery techniques, such as electroporation and chemical transformation, conjugation eliminates the need for DNA extraction, thereby preventing DNA damage during isolation. While most established conjugation protocols allow for DNA transfer in liquid media or on a solid surface, we developed a procedure for conjugation within solid media. Such a protocol may expand conjugation as a tool for DNA transfer to species that require semi-solid or solid media for growth. Conjugation within solid media could also provide a more stable microenvironment in which the conjugative pilus can establish and maintain contact

SUBMITTER: Soltysiak MPM 

PROVIDER: S-EPMC6829330 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature