Effects of Membrane and Biological Target on the Structural and Allosteric Properties of Recoverin: A Computational Approach.

ABSTRACT: Recoverin (Rec) is a prototypical calcium sensor protein primarily expressed in the vertebrate retina. The binding of two Ca2+ ions to the functional EF-hand motifs induces the extrusion of a myristoyl group that increases the affinity of Rec for the membrane and leads to the formation of a complex with rhodopsin kinase (GRK1). Here, unbiased all-atom molecular dynamics simulations were performed to monitor the spontaneous insertion of the myristoyl group into a model multicomponent biological membrane for both isolated Rec and for its complex with a peptide from the GRK1 target. It was found that the functional membrane anchoring of the myristoyl group is triggered by persistent electrostatic protein-membrane interactions. In particular, salt bridges between Arg43, Arg46 and polar heads o

SUBMITTER: Borsatto A 

PROVIDER: S-EPMC6829511 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature