Obtaining Genome Sequences of Mutualistic Bacteria in Single Microcystis Colonies.

ABSTRACT: Cells of Microcystis are associated with heterotrophic bacteria and organized in colonies in natural environment, which are basic elements in the mass occurrence of cyanobacterial species. Analyzing these colonies by using metagenomics is helpful to understand species composition and relationship. Meanwhile, the difference in population abundance among Microcystis colonies could be used to recover genome bins from metagenome assemblies. Herein, we designed a pipeline to obtain high-quality genomes of mutualistic bacteria from single natural Microcystis colonies. Single colonies were lysed, and then amplified by using multiple displacement amplification to overcome the DNA quantity limit. A two-step assembly was performed after sequencing and scaffolds were grouped into

SUBMITTER: Tu J 

PROVIDER: S-EPMC6829522 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature